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UNIT 1:  CELLULAR FUNCTIONS

  The Structure and Function of Cells

The functional unit of any living organism is the cell.  The functional units that make up any cell are called organelles.  All cells, from bacteria to giant squid cells, contain the same basic parts or organelles.  The difference is that some cells contain more or less of certain organelles, depending on their function.  For example, sperm cells contain a lot of mitochondria so that they can produce large amounts of ATP energy for locomotion, while pancreatic cells contain a lot of Golgi bodies since they produce and package large amounts of protein – insulin.  All cells contain the same basic parts, yet each type possesses its own unique ratio of each organelle.

  A.  The Discovery of Cells

  ·         Robert Hooke was the first to observe cells in cork tissue

·         following his initial observations, Schleiden, Schwann, and Virchow (Figure 2.2, p. 38) each made contributions to the development of the cell theory:

-          all living things are composed of cells

-          cells are the basic units of living organisms

-          all cells come from pre-existing cells

·         Schleiden first observed that all plant tissue was made of cells

·         Schwann then extended this observation to animal tissue, and eventually all living things

·         Virchow furthered both discoveries by claiming that cells could only arise from pre-existing cells, which laid to rest the theory of spontaneous generation – living organisms can be generated from non-living matter

  CELL SIZE AND SHAPE

  ·         photomicrographs – photos of cells, indicate that most cells (plant or animal) are anywhere from 10 to 100 µm long (Figure 2.4, p. 39 illustrates the relative sizes of various biological molecules and organisms)

·         any cell will grow to a particular size until its large volume, compared to its cell membrane surface area, reduces both its ability to take in nutrients, water, and other materials, and its ability to eliminate waste products effectively

·         all of what enters and exits a cell must go through the cell membrane

·         a smaller cell has a greater cell membrane surface area compared to its volume than a larger cell

·         if a cell gets too big, its cell membrane area cannot absorb or eliminate enough material efficiently to sustain its large size

·         Table 2.1, p. 40 demonstrates the effect that size has on surface area to volume ratio – not that the smaller cube possesses the largest S.A. to vol. ratio

  Homework:  p. 40, 1-4

  B.  Cell Structure and Function

  1.     Prokaryotes &. Eukaryotes:  Two Major Categories of Cells

          PROKARYOTES

·         lack internal compartments and membrane-bound organelles

·         prokaryotic are only comprised of unicellular organisms like all the bacteria, all the cells that belong to the kingdoms Archaebacteria and Eubacteria

          EUKARYOTES

·         “eu”, meaning “good” in Greek, refers to the fact that eukaryotic cells have a good or true nucleus, as well as other compartmentalized and membrane-bound structures

·         these kinds of cells can be found in both unicellular organisms – like Protists (Paramecium, Amoeba, and Euglena)

·         the compartmentalized, membrane-bound structures are called organelles, each having its own specific function

·         for a detailed difference between these two types of cells, see Figure 2.5, p. 41, or see http://www.cat.cc.md.us/courses/bio141/lecguide/unit1/proeu/proeu.html

2.   Non-Cytoplasmic Organelles and Their Function

          CELL (PLASMA) MEMBRANE

  ·         this structure effectively separates the cytoplasm and all its constituents from a cell’s external environment

·         it has an extremely crucial role in controlling what enters (nutrients, oxygen, etc.) and what exits (cellular waste, carbon dioxide, etc.) a cell

·         the actual membrane itself, as well as the components that the membrane accommodates, both have a large impact on the cell’s survival

·         the plasma membrane’s architecture is illustrated in Figure 2.6, pp. 42-43 – referred to as the fluid mosaic model

·         the four main constituents of the membrane that contribute to its proper functioning are the:

o        phospholipids – amphophilic molecules

o        cholesterols – maintain membrane fluidity

o        proteins    – integral (intrinsic) proteins form channels to facilitate movement into and out of cells

-- extrinsic proteins have attachments that help maintain the structure of the cell (i.e. cytoskeleton), or serve 

   as recognition sites for cell to cell communication

o        glycocalyx – binding sites or recognition sites for messenger molecules, as well as lubrication and adhesion to cells

          CELL WALL

  ·         not found in animal cells

·         found in Archaebacteria, Eubacteria, some protists, fungi, and all plant cells

·         made of cellulose – tough polysaccharide

·         the cell membrane presses up against the cell wall, giving plant tissue its rigidity and ability to stand upright, against gravity’s forces

·         since the cell wall is resistant, it helps to structurally support the cell

·         the membrane pushes on the cell wall like a balloon would push up against pantyhose if it were placed inside them and blown up

·         a cell membrane will never burst, despite being stretched by large amounts of water pressure, as long as it is contained by its cell wall

·         cell walls play a role in both living and dead cells

·         the strength of wood, which is mostly dead cells, arises from the fact that it consists of lignified cell walls

·         when penicillin is added to a bacteria culture, it kills it because it prevents the bacteria from growing a cell wall

·         therefore, when a bacteria absorbs water, and its cell membrane expands, the entire cell will explode, since no cell wall is there to resist the expansion

          NUCLEUS

  ·         this spherical shaped organelle is considered to be the genetic control centre of the cell

·         the most obvious organelle seen when viewing a cell

·         in eukaryotes, it contains the DNA in a form called chromatin – thickens during cell division to become chromosomes

·         the coating on the nucleus – which protects and controls the passage of materials into and out of the nucleus, is called the nuclear envelope (see Figure 2.9, p. 46)

·         the nuclear envelope has nuclear pores (Figure 2.10, p. 48) to allow substances like messenger proteins, mRNA, and other important materials to enter and exit the nucleus

·         the nucleus also contains another structure called the nucleolus – a dark, dense structure that is composed of DNA, granules, and protein fibres (see Figure 2.10, p. 48)

·         the nucleolus is responsible for making ribosomes – proteins that are crucial in the construction of polypeptide chains during protein synthesis

          CYTOPLASM

·         a highly organized area of matter, between the nucleus and the cell membrane of a cell, that contains all the organelles, as well as the fluid called cytosol – contains a concentrated mix of ions and molecules such as enzymes, amino acids, ATP, carbohydrates, oxygen, and carbon dioxide

  Homework:  p. 47, 1-6

  3.   Cytoplasmic Organelles and Their Function

          VACUOLES AND VESSICLES

  ·         vacuoles are basically phospholipid membrane bags of water and dissolved molecules

·         they are surrounded by a single-layered membrane called a tonoplast, and they’re found mainly in plant cells to store water (for photosynthesis and hydroskeleton support) and to store starch molecules

·         vessicles are used for transporting materials from one area of the cell to another – kind of like the cell’s “in-house” courier service moves materials to from one organelle to another

·         Figure 2.10, p. 48, V, shows a vessicle approaching the nucleus

           RIBOSOMES

  ·         these are dense granules that can be found on the surface of endoplasmic reticulum or scattered throughout the cytoplasm

·         they consist of a combination of RNA and protein

·         their function is to assemble amino acids together in protein synthesis

          ENDOPLASMIC RETICULUM

  ·         referred to as ER, this is a series of interconnected small tubes made of membranes that branch out from the nuclear envelope (see Figure 2.11, p. 49)

·         part of the ER may contain ribosomes, which gives in a “rough” appearance – therefore, E.R. with ribosomes is called rough endoplasmic reticulum (RER)

·         protein synthesis of proteins that are most likely destined for use outside the cell, are synthesized on the RER (see Figure 2.12 b, p. 49)

·         smooth endoplasmic reticulum (SER) does not contain ribosomes (Figure 2.12 a, p. 49)

·         the function of the SER is to make lipids – typically phospholipids and steroids, and to store calcium ions

·         it is common to find a lot of SER in the testes and the ovaries, both tissue cells where high levels of steroid hormones production occur

          GOLGI COMPLEX

  ·         very important to the operation of the cell

·         they appear to be flattened stacks of membrane that function to receive, modify, and transport proteins produced by the ER

·         if the proteins are to be used outside the cell, the Golgi complexes package them into vessicles and send them to the cell membrane for export out of the cell (see Figure 2.13, p. 50)

          LYSOSOMES

  ·         these are made by both the Golgi complex and the ER

·         they are membrane-bound sacs that form digestive compartments

·         they possess hydrolitic enzymes – substances that aid in catabolic reactions

·         unicellular organisms use them to break down food

·         the white blood cells of humans, namely the neutrophils and macrophages) contain many lysosomes to engulf and destroy invading bacteria

·         lysosomes are also referred to as “suicide bags” – they digest damaged or old parts of the cell that they are a part of, to help the cell recycle some of the molecules into forming new organelles  (see Figure 2.14, p. 51)

·         research has linked the accumulation of the undigested material in lysosomes over time to ageing

·         when lysosomes lack the enzymes they should have, it could lead to diseases known as lysosomal storage diseases

o        if lysosomes in human brain cells are missing necessary digestive enzymes that normally break down excess fat in the brain, it can cause a lysosomal storage disease called Tay-Sachs disease -- a hereditary condition that results in the deterioration of the brain -- humans with Tay-Sachs disease don’t live past five years old!

o        some other lysosomal diseases are gangliosidosis, Sly syndrome, and Hurler syndrome

o        there are approximately 30 human diseases linked to lysosomal dysfunction, which clearly indicates the importance of this organelle to the cell

·         three examples of lysosomal tissue digestion are the loss of the tail in a tadpole, the loss of unwanted tissue during insect metamorphosis, and the loss in tissue between human fingers during embryo development

          MITOCHONDRIA

  ·         found in plant and animal cells

·         they are the main site of cellular respiration – an extremely important process that produces ATP (the cell’s “gasoline”) – by extracting energy from glucose

·         this process will be studied in detail in SBI 4U1

·         the structure of the mitochondrion is illustrated in Figure 2.16, p. 53  --  YOU MUST KNOW IT!!  

        CHLOROPLAST

·         found only in photosynthetic organisms

·         their structure is illustrated in Figure 2.17, p. 54  --  YOU MUST KNOW IT!!

·         they contain chlorophyll – a pigment that absorbs the sun’s photonic energy and uses it to fix/incorporate CO2 into glucose, in a process known as photosynthesis

  ·         Dr. Lynn Margulis, a professor of botany at the University of Massachusetts , proposed a theory that mitochondria and chloroplasts were, at one point in evolution (about 1.4 billion years ago), free living bacterial cells and algal cells respectively

·         the theory suggests that larger cells engulfed these smaller organisms, and instead of destroying them, they harbored them for their beneficial properties

·         the evidence to support this theory is that…

o        mitochondria and chloroplasts have their own DNA and ribosomes, therefore they reproduce on their own, separately from the cell

o        both mitochondria and chloroplasts are about the same size as bacteria

o        recent discoveries have found that some amoebas that are infected with bacteria actually survive, while still harboring over 40 000 of them within their bodies, and once the bacteria are removed the amoebas die, proving that it is possible for an organism to become dependent on an invading organism, and that it is possible for both invader and host to co-exist

  CYTOSKELETON

  ·         the support system that maintains the shape of animal cells

·         consists of a supportive network of fine protein fibres – microfilaments, intermediate filaments, and microtubules that support the cell, help anchor the organelles in place throughout the cell, and play a role in relaying messages back and forth between the cell membrane and the interior of the cell

          CILIA AND FLAGELLA

  ·         fine protein fibres that have a locomotive function for some cells

·         flagella are long and less numerous, while cilia are short and more numerous

·         cilia surround a Paramecium to help it swim in its environment

·         human sperm cells use a flagellum in a whip-like manner to swim to the egg during fertilization (see Figure 2.9, p. 56)

  Homework:  p. 56, 1-7.

  ·         for a more detailed description of the structure and function of cell organelles, see http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookCELL2.html#The%20Cell%20Membrane, and click on the appropriate organelle.